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High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

DOI:
10.3791/52195-v

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09:22 min

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November 27, 2014

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,
1Department of Pharmacology,University of Minnesota, 2Department of Surgery,University of Minnesota, 33M Corporate Research Laboratory

Capitoli

  • 00:05Titolo
  • 01:38Plating and Treating Cells
  • 02:16Opsonization of Flourescent Particles
  • 03:36Phagocytosis
  • 05:08Actin Staining
  • 06:10Quantification
  • 07:33Results: Confocal Microscopy of Phagocytosis and Actin Polymerization
  • 08:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

Tags

Keywords: Fluorometric AnalysisPhagocytosisActin PolymerizationHigh ThroughputMacrophagesFluorescent ParticlesTrypan BlueDAPIFlow CytometryCellular Processes

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