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A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

DOI:
10.3791/52196-v

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11:20 min

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December 03, 2014

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,
1Department of Neurobiology and Behavior,Cornell University, 2Department of Biomedical Engineering,Cornell University

Capitoli

  • 00:05Titolo
  • 01:56Preparing the Mouse for Surgery
  • 02:56Expose the Dorsal Laminae of Thoracic Vertebrae
  • 05:11Perform a Dorsal Laminectomy
  • 06:54Seal the Chamber
  • 09:16Results: Chronic Spinal Chamber Allows Repeated Multi-photon Imaging
  • 10:56Conclusion

Summary

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In this video, we describe a procedure for implanting a chronic optical imaging chamber over the dorsal spinal cord of a live mouse. The chamber, surgical procedure, and chronic imaging are reviewed and demonstrated.

Tags

Keywords: Spinal ChamberIn Vivo ImagingMouse Spinal CordSurgical ProcedureOptical AccessNonlinear MicroscopyNeurodegenerative InsultsChronic ImagingVertebral ClampingSurgical ImplantPost-operative CareImaging Stability

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