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Desarrollo de una<em> In vitro</em> Sistema modelo para estudiar la interacción de<em> Equus</em><em> Caballus</em> IgE con su receptor de alta afinidad Fc RI
JoVE Journal
Immunologia e infezioni
Author Produced
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JoVE Journal Immunologia e infezioni
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
DOI:

07:31 min

November 01, 2014

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Capitoli

  • 00:00Titolo
  • 00:10Introduction
  • 01:14Animation
  • 02:37Washing
  • 03:22Antigen Challenge
  • 03:48Substrate
  • 04:23Stopping the Reaction
  • 04:47Reading
  • 04:54Calculations
  • 06:22Risultati
  • 07:02Conclusion

Summary

Traduzione automatica

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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