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Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
JoVE Journal
Medicina
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JoVE Journal Medicina
Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
DOI:

11:31 min

November 30, 2015

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Capitoli

  • 00:05Titolo
  • 02:09Serum, DNA, and PBMC Isolation Stage 1
  • 03:49DNA Isolation Stage 1
  • 04:36Leukocyte RNA Isolation Stage 1
  • 06:02PBMC Isolation Stage 2
  • 08:06Serum Isolation Stage 2 and PBMC Isolation Stage 3
  • 10:30Results: Ideal Leukocyte RNA Processing Yields
  • 10:50Conclusion

Summary

Traduzione automatica

We outline a methodology for the processing of whole blood to obtain a variety of components for further analysis. We have optimized a streamlined protocol that enables rapid, high-throughput simultaneous processing of whole blood samples in a non-clinical setting.

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