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Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury

Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury

DOI:
10.3791/52228-v

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08:43 min

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November 23, 2014

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, , ,
1Department of Neurosciences,Case Western Reserve University, 2Department of Biomedical Engineering,Case Western Reserve University, 3Department of Pediatrics,Case Western Reserve University

Capitoli

  • 00:05Titolo
  • 01:10Dorsal Column Crush Injury
  • 04:00Intravital Imaging
  • 06:51Results: Post–injury Observations
  • 08:18Conclusion

Summary

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Two-photon intravital imaging can be used to investigate interactions among different cell types in the spinal cord in their native tissue environment in a bone marrow chimeric animal with a dorsal column traumatic spinal cord crush injury.

Tags

Intravital ImagingAxonal InteractionsMicrogliaMacrophagesMouseDorsal Column Crush InjurySpinal Cord InjuryTwo-photon MicroscopyBone Marrow ChimeraLaminectomy

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