Geração de plasmídeo vectores que expressam proteínas marcadas-bandeira nos termos do regulamento de Alongamento Human Fator-1α Promotor Usando Assembleia Gibson
Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.