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3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii
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Neuroscienze
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JoVE Journal Neuroscienze
3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii

3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii

DOI:
10.3791/52237-v

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06:33 min

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December 09, 2014

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,
1Bio5 Institute,University of Arizona, 2Department of Neurology,University of Arizona, 3Department of Immunobiology,University of Arizona

Capitoli

  • 00:05Titolo
  • 01:35Brain Sectioning
  • 02:37Optical Clearing of Brain Tissue Sections
  • 03:56Mounting Brain Tissue Sections
  • 04:59Results: Toxoplasma gondii Cysts Reside within Distant Neuronal Processes
  • 06:00Conclusion

Summary

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Using this protocol, we were able to image 160 µm thick brain sections from mice infected with the parasite Toxoplasma gondii, which enables visualization and analysis of the spatial relationship between the encysting parasite and the infected neuron.

Tags

3-D ImagingNeuronsToxoplasma GondiiIntracellular ParasiteBrain InfectionCentral Nervous System (CNS)Neuron InfectionPersistent InfectionThree-dimensional VisualizationImmunostainingElectron MicroscopySerial Sectioning

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