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神经元的3-D成像和分析感染<em>在体内</em>与<em>弓形虫</em
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Neuroscienze
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JoVE Journal Neuroscienze
3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii
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神经元的3-D成像和分析感染<em>在体内</em>与<em>弓形虫</em

DOI:
10.3791/52237-v

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06:33 min

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December 09, 2014

•

,
1Bio5 Institute,University of Arizona, 2Department of Neurology,University of Arizona, 3Department of Immunobiology,University of Arizona

Capitoli

  • 00:05Titolo
  • 01:35Brain Sectioning
  • 02:37Optical Clearing of Brain Tissue Sections
  • 03:56Mounting Brain Tissue Sections
  • 04:59Results: Toxoplasma gondii Cysts Reside within Distant Neuronal Processes
  • 06:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

使用该协议，我们能够从感染寄生虫弓形虫 ，使可视化和的encysting寄生虫和感染的神经元之间的空间关系分析的小鼠图像160微米厚的脑切片。

Tags

Keywords: 3-D ImagingNeuronsToxoplasma GondiiIntracellular ParasiteBrain InfectionCentral Nervous System (CNS)Neuron InfectionPersistent InfectionThree-dimensional VisualizationImmunostainingElectron MicroscopySerial Sectioning

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