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Cultura da embrionárias mouse cocleares explantes e Gene Transferência de Eletroporação
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Capitoli
- 00:05Titolo
- 01:51Dissection of the Inner Ears
- 02:56Gerneration of Organ of Corti Explant Cultures
- 05:25Electroporation-mediated Gene Transfer
- 07:34Results: Electroporation of Atoh1.EGFP and NeuroD1 into the Organ of Corti Explant Culture
- 08:32Conclusion
We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.
