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TIRFM والحساسة للدرجة الحموضة GFP-تحقيقات في تقييم ناقل عصبي الحويصلة حيوية في خلايا SH-SY5Y العصبية: التصوير خلية وتحليل البيانات
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Capitoli
- 00:05Titolo
- 03:32Cell Imaging by Total Internal Reflection Fluorescence Microscopy (TIRFM)
- 06:08Fluorescence Intensity Quantification
- 07:09Photobleaching Correction and Threshold Determination
- 08:16Selection of Fusion Events Using a Semiautomatic Procedure
- 10:01Data Analysis
- 11:31Results: Analysis of Synaptic Vesicles Fusion Under Resting and Stimulated (Potassium-induced Depolarization) Conditions Are Shown
- 12:37Conclusion
This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.
