È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie,Université Pierre et Marie Curie, 2Kavli Institute for Brain and Mind,University of California, San Diego, 3Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke,National Institute of Health
Capitoli
- 00:05Titolo
- 01:44Giant Unilamellar Vesicle Growth by Electroformation
- 04:29Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
- 06:07Harvesting and Transferring GUVs
- 07:33Patch-clamping GUVs
- 09:14Results: Microscopy and Patch Clamp Studies of KvAP GUVs
- 11:03Conclusion
Genoprettelse af det transmembrane protein, KvAP, i gigantiske unilamelblærer (GUVs), er dokumenteret i to dehydrering-rehydrering metoder - electroformation og gel-assisteret hævelse. I begge fremgangsmåder er små unilamellare vesikler indeholdende proteinet fusioneret sammen for at danne GUVs, som derefter kan studeres ved fluorescensmikroskopi og patch-clamp elektrofysiologi.
Tags
Keywords:
Transmembrane ProteinVoltage-gated Ion ChannelKvAPGiant Unilamellar Vesicles (GUVs)Biomimetic SystemMembrane Associated PhenomenaDehydration-rehydrationElectroformationGel-assisted SwellingSmall Unilamellar VesiclesRehydration BufferAC FieldAgarose GelFluorescence MicroscopyPatch-clampProtein Distribution
