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1Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie,Université Pierre et Marie Curie, 2Kavli Institute for Brain and Mind,University of California, San Diego, 3Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke,National Institute of Health
Capitoli
- 00:05Titolo
- 01:44Giant Unilamellar Vesicle Growth by Electroformation
- 04:29Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
- 06:07Harvesting and Transferring GUVs
- 07:33Patch-clamping GUVs
- 09:14Results: Microscopy and Patch Clamp Studies of KvAP GUVs
- 11:03Conclusion
Elektroformation, und Gel-unterstützte Schwellung - Die Wiederherstellung der Transmembranprotein, KvAP, in riesige unilamellare Vesikel (GUVs) ist für zwei Dehydration-Rehydrierung Methoden nachgewiesen. In beiden Verfahren werden kleine unilamellare Vesikel, die das Protein enthält, zusammengeschmolzen, um GUVs die dann durch Fluoreszenzmikroskopie und Patch-Clamp-Elektrophysiologie untersucht werden können zu bilden.
Tags
Keywords:
Transmembrane ProteinVoltage-gated Ion ChannelKvAPGiant Unilamellar Vesicles (GUVs)Biomimetic SystemMembrane Associated PhenomenaDehydration-rehydrationElectroformationGel-assisted SwellingSmall Unilamellar VesiclesRehydration BufferAC FieldAgarose GelFluorescence MicroscopyPatch-clampProtein Distribution
