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Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
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Neuroscienze
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JoVE Journal Neuroscienze
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

DOI:
10.3791/52299-v

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08:42 min

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December 27, 2014

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,
1Pharmacology and Toxicology,Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin

Capitoli

  • 00:05Titolo
  • 01:24Preparing the Nasal Septum
  • 03:12Isolating the Olfactory Epithelium
  • 05:01Staininig the Epithelium
  • 06:53Results: Images of Olfactory Epithelium
  • 08:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.

Tags

Keywords: Whole Mount LabelingCiliaMain Olfactory SystemMiceOlfactory Sensory NeuronsDendritic KnobOlfactory Signal TransductionKirrel2Axon GuidanceAdhesion MoleculeActivity-dependent Localization

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