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Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells
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JoVE Journal Medicina
Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells

Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells

DOI:
10.3791/52343-v

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08:48 min

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January 04, 2015

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1Department of Otolaryngology,Vanderbilt University Medical Center

Capitoli

  • 00:05Titolo
  • 01:22Preparation of Glass Coverslips for Polyacrylamide Gels
  • 02:59Preparation of Polyacrylamide Gels for Invadopodia Assays
  • 05:09Preparation of Extracellular Matrix for Invadopodia Assays
  • 07:10Results: Invadopodia and Traction Force Assays
  • 08:24Conclusion

Summary

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Mechanical rigidity in the tumor microenvironment plays a crucial role in driving malignant behavior by increasing invadopodia activity and actomyosin contractility. Using polyacrylamide gels (PAAs), invadopodia and traction force assays can be utilized to study the invasive and contractile properties of cancer cells in response to matrix rigidity.

Tags

Keywords: Polyacrylamide GelsInvadopodiaTraction ForceCancer CellsExtracellular Matrix (ECM)ActinActomyosin ContractilityRigidityMigrationInvasionProteolytic DegradationContractile ForcesIn Vitro Assays

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