/
/
Métodos para avaliar a citotoxicidade e Imunossupressão de combustível tabaco Preparações Produto
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:40Intracellular Staining and Flow Cytometry
- 03:52K562 Killing Assay
- 06:33Results: Representative Cytotoxic and Immunosuppressive Effects of Combustible Tobacco Product Preparations
- 08:42Conclusion
Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
