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一个<em>在体外</em>酶测定来测量转录抑制作用镓（Ⅲ）和H<sub> 3</sub> -5,10,15-三（五氟苯基）corroles
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An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
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一个<em>在体外</em>酶测定来测量转录抑制作用镓（Ⅲ）和H<sub> 3</sub> -5,10,15-三（五氟苯基）corroles

DOI:
10.3791/52355-v

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09:00 min

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March 18, 2015

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, , , , , ,
1Division of Chemistry and Chemical Engineering,California Institute of Technology, 2Department of Molecular Medicine,Beckman Research Institute of the City of Hope

Capitoli

  • 00:05Titolo
  • 01:44RNA Transcription with Corrole Treatment
  • 03:09Agarose Gel Electrophoresis (1% Agarose Gel)
  • 05:58RNA Quantification via UV-Vis Spectroscopy
  • 06:47Results: Assessment of RNA Transcription by Agarose Gel Electrophoresis and UV-Vis Spectroscopy
  • 08:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

镓（Ⅲ）5,10,15-（三）pentafluorophenylcorrole和其游离碱类似物显示出低的微摩尔细胞的细胞毒性。这个手稿描述的RNA转录反应中，成像的RNA用溴化乙锭染色的凝胶，并用UV-Vis光谱进行定量的RNA，为了通过corroles评估转录抑制和演示评价抗癌候选属性的一个简单的方法。

Tags

Transcription InhibitionRNA Transcription AssayGallium(III) 51015-(tris)pentafluorophenylcorrole51015-(tris)pentafluorophenylcorroleActinomycin DTriptolideAnticancer Drug Mechanism Of Action

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