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A Multi-detection Assay for Malaria Transmitting Mosquitoes
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Biologia
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JoVE Journal Biologia
A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

DOI:
10.3791/52385-v

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09:00 min

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February 28, 2015

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, , , ,
1Department of Pathology, Microbiology and Immunology,School of Veterinary Medicine, University of California – Davis, 2Veterinary Genetics Laboratory,School of Veterinary Medicine, University of California, Davis

Capitoli

  • 00:05Titolo
  • 01:27PCR Amplification and SNP Extension
  • 04:01Conditioning the SNP Extension Product to Optimize Mass Spectrometry Analysis
  • 05:15MALDI-TOF Mass Spectrometry
  • 06:36Results: Malaria Transmitting Mosquitoes
  • 08:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

Malaria transmitting mosquitoes have a number of epidemiologically important characteristics that can only be detected using molecular techniques. Utilizing a MALDI-TOF based SNP genotyping platform, we developed an assay for simultaneously detecting multiple key traits (species, insecticide resistance, parasite infection and host choice) of malaria vectors.

Tags

Keywords: Multi-detection AssayAnopheles GambiaeMalariaMosquitoesSpecies IdentificationInsecticide ResistancePlasmodium DetectionHost PreferencePCRELISAMultiplex Genotyping

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