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A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
DOI:

07:29 min

December 06, 2014

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Capitoli

  • 00:05Titolo
  • 01:47Preparation of the Tissue Homogenate
  • 04:15Separation and Clarification of the Tissue Supernatant
  • 05:37Results: Tumor Infiltrating Lymphocyte (TIL) Subsets in the Homogenate
  • 07:00Conclusion

Summary

Traduzione automatica

This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.

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