/
/
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 02:34Magnetic Bead-based Exosome Extraction
- 04:55Precoating of the Electrode with a Specific Capture Probe
- 06:16Electric Field-induced Release of Exosome Cargo
- 07:22Exosome Cargo Detection and Readout
- 08:25Results: Validation of EFIRM and Detection of the Human CD63-GFP in Mouse Serum and Saliva
- 09:41Conclusion
Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.
