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In vitro berikelse for eggstokkreft Tumor-initiere Cells
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Medicina
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JoVE Journal Medicina
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

In vitro berikelse for eggstokkreft Tumor-initiere Cells

DOI:
10.3791/52446-v

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11:37 min

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February 18, 2015

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, ,
1Women’s Malignancies Branch,National Cancer Institute

Capitoli

  • 00:05Titolo
  • 01:43Multicellular Spheroid Generation from Ovarian Cancer Cell Lines
  • 05:02Cell Staining for Flow Cytometric Confirmation of Tumor-initiating Cells (TIC) Markers
  • 07:39Confirmation of Tumorigenicity by In vivo Subcutaneous Injections of TIC Cultures
  • 09:03Results: Low-attachment, Serum-free Conditions Enrich for Cells with TIC Markers and Tumorigenicity
  • 11:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

Tumor-initiating cells (TICs) may represent a viable therapeutic target for the treatment of ovarian cancer, a highly recurrent and fatal disease. We present a protocol for culture conditions that enrich for this highly tumorigenic population of cells.

Tags

Keywords: Ovarian CancerTumor-initiating CellsTICsIn Vitro EnrichmentSphere FormationTumorigenicityStem Cell MarkersCD133ALDHLow AttachmentSerum-free MediaGrowth FactorsAdherent CellsNon-adherent CellsFloater CellsSignaling PathwaysDrug Targeting

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