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Capitoli
- 00:05Titolo
- 02:01Intravitreal Injection of AAV
- 03:52Immunohistochemistry: Retinal Cryosections
- 05:04Immunohistochemistry: Retinal Flatmounts
- 06:15Immunohistochemistry: Labeling, Mounting, and Imaging
- 07:24PCR Analysis of Mouse Blood Samples
- 08:24Results: Retinal Permeability in Dp71-null Mice
- 09:40Conclusion
To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability.
