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Proteinoprensning Teknik der tillader detektering af Sumoylation og Ubiquitinering af Spirende Gær Kinetochore Proteiner Ndc10 og Ndc80
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Biologia
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JoVE Journal Biologia
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Proteinoprensning Teknik der tillader detektering af Sumoylation og Ubiquitinering af Spirende Gær Kinetochore Proteiner Ndc10 og Ndc80

DOI:
10.3791/52482-v

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12:28 min

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May 03, 2015

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, ,
1Genetics Branch, Center for Cancer Research,National Cancer Institute, National Institute of Health

Capitoli

  • 00:05Titolo
  • 01:48Extraction of Proteins
  • 04:54Purification of HF-Smt3 Conjugates
  • 07:31Immunoprecipitation of Myc-tagged Kinetochore Proteins
  • 09:52Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in S. cerisiae
  • 11:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

Dette manuskript beskriver påvisning af sumoylation og ubiquitinering af kinetochore proteiner, Ndc10 og Ndc80, i spirende gæren Saccharomyces cerevisiae.

Tags

Protein PurificationSumoylationUbiquitinationKinetochore ProteinsNdc10Ndc80Post-translational ModificationsSaccharomyces Cerevisiae

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