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斑马鱼角膜植研究集体细胞迁移和再上皮化在皮肤伤口愈合
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
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斑马鱼角膜植研究集体细胞迁移和再上皮化在皮肤伤口愈合

DOI:
10.3791/52489-v

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09:35 min

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February 25, 2015

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, , , , ,
1Biomedical Sciences Program,Midwestern University, 2Department of Microbiology & Immunology,Midwestern University

Capitoli

  • 00:05Titolo
  • 01:55Preparation of Anesthesia, Equipment, and Media
  • 02:55Establish Explant Cultures
  • 04:40Brightfield and Video Microscopy
  • 05:20Fixation for Immunofluorescence Microscopy
  • 07:02Results: Cell Migration in Zebrafish Keratocytes
  • 08:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

斑马鱼角膜基质细胞的表外植迁移，并提供了一个体外模型集体细胞迁移的上皮伤口愈合的情况下机制的研究。这些协议的细节，以建立初级外植体培养在集体细胞迁移测定法使用的有效方式。

Tags

ZebrafishKeratocyteExplantCollective Cell MigrationReepithelializationCutaneous Wound HealingSingle Cell MigrationActin-rich LamellaeActin CableEpithelial To Mesenchymal TransitionGene ExpressionMutantTransgenicIn Vitro Model

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