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En guide til å generere og bruke hiPSC Avledet NPCer for studier av nevrologiske sykdommer
JoVE Journal
Medicina
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JoVE Journal Medicina
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
DOI:

09:30 min

February 21, 2015

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Capitoli

  • 00:05Titolo
  • 01:22Harvest of Neural Rosettes
  • 03:03Expansion of Neural Progenitor Cells
  • 04:27NPC Transduction
  • 05:15Neurosphere Migration Assay
  • 07:06Results: Patient-specific hiPSCs can be Differentiated into Healthy NPCs and Neurons
  • 08:56Conclusion

Summary

Traduzione automatica

This protocol describes how neural progenitor cells can be differentiated from human induced pluripotent stem cells, in order to yield a robust and replicative neural cell population, which may be used to identify the developmental pathways contributing to disease pathogenesis in many neurological disorders.

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