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Manipulation und<em> In-vitro-</em> Ausbau der<em> Xenopus laevis</em> Eizellen, von Intrazytoplasmatische Spermieninjektion gefolgt, um embryonale Entwicklungsstudie
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
DOI:

09:22 min

February 09, 2015

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Capitoli

  • 00:05Titolo
  • 01:14Preparations
  • 03:47Injecting Oocytes with Oligos and Oocyte Maturation
  • 05:55Intracytoplasmic Sperm Injection
  • 08:12Results: ICS Embryo Survival
  • 09:01Conclusion

Summary

Traduzione automatica

We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

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