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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
 

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

Article DOI: 10.3791/52497-v • 09:18 min • May 27th, 2015
May 27th, 2015 •
1, 1
1Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago

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Listeria monocytogenes causes fetal infections in pregnant women and meningitis in susceptible populations. Subpopulations of bacteria can colonize cardiac tissue, causing myocarditis in patients and laboratory animals. Here we present a protocol that describes how to assess L. monocytogenes cardiac cell invasion in vitro and cardiac colonization in infected animals.

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Bacterial Invasion Cardiac Cells Culture Heart Colonization Listeria Monocytogenes Gram-positive Intracellular Pathogen Invasive Infections Immunocompromised Patients Meningitis Encephalitis Fetal Abortion Cardiac Illness Colonization Of Cardiac Tissue Pathologies Cardiotropism Assessment In Vitro Methods In Vivo Methods H9c2 Rat Cardiac Myoblasts Bacterial Colonization Infected Mice
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