Rivista
/
Biologia
/
Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

DOI:
10.3791/52523-v

•

08:36 min

•

March 25, 2015

•

, , , , , ,
1Division of Biology,Kansas State University, 2Department of Medical and Molecular Genetics,Indiana University School of Medicine, 3Eck Institute for Global Health,University of Notre Dame, 4Department of Biological Sciences,University of Notre Dame, 5Department of Entomology,Kansas State University

Capitoli

  • 00:05Titolo
  • 01:39Preparing Chitosan/Interfering RNA Nanoparticles
  • 04:05Preparing Mosquito Food Containing Chitosan/Interfering RNA Nanoparticles
  • 05:07Feeding Mosquito Larvae with Food Containing Chitosan/Interfering RNA Nanoparticles
  • 07:07Results: Changes in Mosquito Development Following Chitosan/Interfering RNA Targeting of Larval Genes
  • 08:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by larvae.

Tags

Keywords: ChitosanInterfering RNANanoparticleGene SilencingDisease VectorMosquito LarvaeAnopheles GambiaeAedes AegyptiRNA InterferenceDouble-stranded RNASmall Interfering RNA

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/52523?article_type=v

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
check_url/it/52523?article_type=v

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
check_url/it/52523?article_type=v

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
check_url/it/52523?article_type=v

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
check_url/it/52523?article_type=v

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
check_url/it/52523?article_type=v

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
check_url/it/52523?article_type=v

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
check_url/it/52523?article_type=v

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
check_url/it/52523?article_type=v

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
check_url/it/52523?article_type=v

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Read Article