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Capitoli
- 00:05Titolo
- 01:45Seeding and Culturing of HL-1 Atrial Myocytes on Coverslips
- 04:12Loading HL-1 Cells with Calcium- and Voltage-sensitive Dyes
- 05:20Dual Channel Optical Mapping of HL-1 Cells
- 06:42Results: Action Potentials and Calcium Signals Propagate Across a Monolayer of HL-1 Atrial Myocytes
- 07:41Conclusion
This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.
