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Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Capitoli
- 00:05Titolo
- 02:17Injection of Thymidine Analogs and Tissue Preparation
- 03:38Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species
- 06:27Imaging and Data Analysis
- 07:05Newborn Hippocampal Cells in Cyclin D2 Deficient Mice
- 08:42Conclusion
This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.
