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フォワード遺伝学は、識別するためのマクロファージを使用した画面<em>トキソプラズマ</emIFN-γ依存性細胞自律イミュニティへの抵抗のための重要な>遺伝子
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Capitoli
- 00:05Titolo
- 02:15Isolation of Parasite Mutants with a Fitness Defect Following Activation of Infected Macrophages
- 06:03Evaluation of Mutants to Determine if the Defect is at the Level of Parasite Survival or Replication Following Activation of Infected Macrophages
- 09:16Results: T. gondii Mutants with Fitness Defects are Identified by Phase Contrast and Immunofluorescence Microscopy
- 10:48Conclusion
Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.
