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앞으로 유전학 식별하는 대 식세포를 사용하여 화면<em> 톡소 포자충</em> 유전자 중요 저항이하는 IFN-γ 의존 세포 자치 내성
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Capitoli
- 00:05Titolo
- 02:15Isolation of Parasite Mutants with a Fitness Defect Following Activation of Infected Macrophages
- 06:03Evaluation of Mutants to Determine if the Defect is at the Level of Parasite Survival or Replication Following Activation of Infected Macrophages
- 09:16Results: T. gondii Mutants with Fitness Defects are Identified by Phase Contrast and Immunofluorescence Microscopy
- 10:48Conclusion
Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.
