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Analyse af Autophagy i<em> Penicillium chrysogenum</em> Ved hjælp Sult Pads i kombination med Fluorescence Microscopy
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Biologia
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JoVE Journal Biologia
Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Analyse af Autophagy i<em> Penicillium chrysogenum</em> Ved hjælp Sult Pads i kombination med Fluorescence Microscopy

DOI:
10.3791/52577-v

•

06:51 min

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February 01, 2015

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1LOEWE Excellence Cluster for Integrative Fungal Research (IPF),Senckenberg Research Institute

Capitoli

  • 00:05Titolo
  • 01:06P. chrysogenum Preparation
  • 02:16Starvation Pad Cultivation Preparation
  • 03:22P. chrysogenum Starvation Pad Inoculation and Incubation
  • 04:25Microscopic Sample Analysis
  • 05:11Results: Representative GFP-SKL Localization
  • 06:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

A convenient and powerful method for studying autophagy in Penicillium chrysogenum by using starvation pads (mixtures of agarose and tap water in a microscope slide containing a central cavity) is presented here.

Tags

AutophagyPenicillium ChrysogenumStarvation PadsFluorescence MicroscopyCellular Quality ControlOrganelle DynamicsProtease ActivityFilamentous FungusMicroscopy Evaluation

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