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JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Upright Imaging of Drosophila Egg Chambers
DOI:

12:29 min

March 13, 2015

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Capitoli

  • 00:05Titolo
  • 01:47Ovariole Dissection
  • 03:25Ovariole Immunofluorescent Staining
  • 05:36Glycerin Jelly Slide Preparation
  • 06:57Egg Chamber Mounting
  • 09:22Egg Chamber Imaging Preparation
  • 10:35Results: Enhanced Imaging with the Upright Technique
  • 11:56Conclusion

Summary

Traduzione automatica

The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

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