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Journal / Environment / EPA Method 1615. Measurement of Enterovirus and…
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Environment

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JoVE Journal Environment
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
 

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Article DOI: 10.3791/52646-v • 12:32 min • January 16th, 2016
January 16th, 2016 •
1, 1, 1, 1, 1, 2
1National Exposure Research Laboratory, U.S. Environmental Protection Agency, 2Technical Services Center, Office of Ground Water and Drinking Water, U.S. Environmental Protection Agency

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Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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EPA Method 1615 Enterovirus Norovirus Water Culture RT-qPCR Virus Detection Reverse Transcription Quantitative Polymerase Chain Reaction Tertiary Concentration Centrifugal Ultrafiltration Standard Curve Reagents Nucleic Acid Extraction Viral Concentrates RNA Dilutions Reverse Transcription Random Primers Reverse Transcriptase Amplification Real-time Thermal Cycler Virus Specific Primers And Probes Genomic Copy Calculations ISO 15216 Risk Assessors Environmental Microbiology Field
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