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Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
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Capitoli
- 00:00Titolo
- 01:34Raising of Zebrafish to Larval Stages
- 02:26Preparation of the Imaging Chamber
- 03:57Mounting and Imaging of the Pre-injured Larvae
- 05:41Amputation Assay
- 06:00Mounting the Larva for Time-lapse Imaging
- 07:28Time-lapse Imaging
- 09:14Data Analysis
- 11:51Representative Results
- 13:11Conclusion
We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
