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Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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JoVE Journal Immunologia e infezioni
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

DOI:
10.3791/52655-v

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13:36 min

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May 06, 2015

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, , ,
1Department of Medicine,University of Alabama at Birmingham, 2Center for AIDS Research,University of Alabama at Birmingham

Capitoli

  • 00:05Titolo
  • 02:31Construction of Modified Plasmid pAd5/H5-HVR1-KWAS-HVR5-His6
  • 05:09Preparation of Modified Ad5 Viral Vector Ad5/H5-HVR1-KWAS-HRV5-His6
  • 09:50Validation of the Rescued Viral Vector
  • 11:22Results: Evaluation of the Divalent Ad5 Vector Ad5/H5-HVR1-KWAS-HVR5-His6
  • 13:02Conclusion

Summary

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Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.

Tags

Keywords: Adenovirus Serotype 5Ad5Antigen Capsid-incorporation StrategyVaccine DevelopmentHIV EpitopeHis6 TagHexonHypervariable RegionNeutralization AssayHumoral ImmunityRare-serotype Adenovirus

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