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Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
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JoVE Journal Medicina
Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

DOI:
10.3791/52656-v

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11:41 min

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March 15, 2015

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, , , , ,
1Department of Pediatrics,Stanford University School of Medicine, 2Department of Orthopaedic Surgery,Stanford University School of Medicine

Capitoli

  • 00:05Titolo
  • 02:00Isolating Tissue Fragments from Femoral Heads
  • 02:40Co-culturing to Study Cell Proliferation
  • 04:19Co-culturing to Study Colonization and Cell Number
  • 06:45Measuring Cell Migration in the Co-culture: Towards Bone Tissue Supernatant
  • 09:30Results: Breast Cancer Cell Proliferation, Colonization, and Migration in Co-culture
  • 11:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

Protocols are described for studying breast cancer cell migration, proliferation and colonization in a human bone tissue explant model system.

Tags

Bone MetastasisBreast CancerIn Vitro ModelMicroenvironmentMetastatic NicheCancer Cell ColonizationBioluminescence ImagingFluorescence MicroscopyFlow Cytometry

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