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Bewertung der Tumor-infiltrierenden Leukozyten-Untergruppen in einer subkutanen Tumormodell
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JoVE Journal Medicina
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Bewertung der Tumor-infiltrierenden Leukozyten-Untergruppen in einer subkutanen Tumormodell

DOI:
10.3791/52657-v

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07:49 min

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April 13, 2015

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, , , ,
1Division of Oncology, Department of Medicine,Washington University School of Medicine, 2Palo Alto Institute for Research and Education,Veterans Affairs Palo Alto Health Care System, 3Laboratory of Immunology and Vascular Biology, Department of Pathology,Stanford University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:29Preparing for Sample Collection and Processing
  • 02:15Tumor Resection and Preparation of Single Cell Tumor Suspension
  • 05:47Results: FACS Staining of Single Cell Suspension and Analysis of Leukocyte Subsets
  • 07:21Conclusion

Summary

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This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.

Tags

Keywords: Tumor-infiltrating LeukocytesTumor MicroenvironmentImmune CellsDendritic CellsRegulatory T CellsMyeloid-derived Suppressor CellsNatural Killer CellsCytotoxic T CellsTumor GrowthTumor SuppressionFlow CytometrySubcutaneous Tumor ModelB16 Melanoma

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