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Oxygène-glucose privation et réoxygénation comme un<em> In Vitro</em> Ischémie-reperfusion modèle pour étudier la dysfonction barrière hémato-encéphalique
JoVE Journal
Medicina
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JoVE Journal Medicina
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
DOI:

08:56 min

May 07, 2015

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Capitoli

  • 00:05Titolo
  • 01:16Seeding Endothelial Cells (RBMECs)
  • 03:31Oxygen and Glucose Deprivation-Reoxygenation In Vitro Model
  • 04:40Immunofluorescence Localization and f-actin Labeling with Rhodamine Phalloidin
  • 07:29An Oxygen-Glucose Deprivation and Reoxygenation (OGD-R) Experiment
  • 08:22Conclusion

Summary

Traduzione automatica

Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.

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