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Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

DOI:

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20:51 min

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December 04, 2007

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Christine Beeton, K. George Chandy

¹Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capitoli

00:00Titolo
00:10Introduction
01:06Cardiac Perfusion
05:36Removing the Brain
09:11Removing the Spinal Cord
11:18Preparation of a Single-Cell Suspension
14:12Percoll Gradient
17:41Harvest of Mononuclear Cells
19:56Conclusion

Summary

Traduzione automatica

In this video we demonstrate how to isolate mononuclear cells from the central nervous system of rats with experimental autoimmune encephalomyelitis.

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