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Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
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Capitoli
- 00:05Titolo
- 01:18Fabrication of Paper Devices
- 04:04Serum and Aqueous Humor Sample Collection and Processing
- 05:09Isolation of Extracellular Vesicles
- 05:56Downstream Assay Example 1: Scanning Electron Micoscopy
- 07:04Downstream Assay Example 2: Paper-based ELISA
- 08:04Downstream Assay Example 3: RNA Isolation
- 10:26Results: Characterization of Captured Extracellular Vesicles
- 11:24Conclusion
This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.
