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Neuroscience

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JoVE Journal Neuroscience
Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
 

Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

Article DOI: 10.3791/52730-v • 12:19 min • June 24th, 2015
June 24th, 2015 •
1, 2, 1
1Department of Neurobiology and Behavior, Stony Brook University, 2Department of Pharmacological Science, Stony Brook University

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We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.

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Nicotine Calcium Signaling Neurotransmitter Release Ventral Hippocampal Axons Pre-synaptic Nicotinic Acetylcholine Receptors Neuromodulation Acetylcholine Synaptic Plasticity Live Imaging Gene-chimeric Preparation VHipp-accumbens Circuit Pre-synaptic Components Post-synaptic Components Genetic Profile Microslices Glutamatergic Synapses Dispersed Neurons NAcc Subtypes Of NAChRs Electrophysiological Recording Live Cell Imaging
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