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Dyrkning Mus hjerteklapper i Miniature Tissue Culture System
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Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System

Dyrkning Mus hjerteklapper i Miniature Tissue Culture System

DOI:
10.3791/52750-v

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08:47 min

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October 19, 2015

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, , , ,
1Department of Molecular Cell Biology,Leiden University Medical Center, 2Department of Engineering Technology,New Jersey Institute of Technology, 3Department of Urology,Leiden University Medical Center, 4Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine,Rutgers New Jersey Medical School

Capitoli

  • 00:05Titolo
  • 01:05Miniature Tissue Culture System (MTCS) Assembly
  • 02:13Mouse Heart Isolation
  • 04:01Mouse Heart Cannulation in the Perfusion Chamber
  • 05:21Mitral and Aortic Valve Ligation and Culture
  • 07:16Results: Histological Analysis of Cultured Valves from 2 Month Old Mice
  • 08:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.

Tags

Keywords: Mouse Cardiac ValvesHeart Valve DiseaseValvular Biology3D OrganizationValvular Endothelial CellsValvular Interstitial CellsEx Vivo Flow ModelMitral ValveAortic ValveValvular CellsSignaling Pathways

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