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Immunologia e infezioni

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使用"桅杆细胞敲击"小鼠分析体内桅杆细胞的功能

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

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使用"桅杆细胞敲击"小鼠分析体内桅杆细胞的功能

DOI:

10.3791/52753-v

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09:07 min

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May 27, 2015

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Nicolas Gaudenzio, Riccardo Sibilano, Philipp Starkl, Mindy Tsai, Stephen J. Galli², Laurent L. Reber

¹Department of Pathology,Stanford University School of Medicine, ²Department of Microbiology & Immunology,Stanford University School of Medicine

Capitoli

00:05Titolo
01:59Generation and Characterization of Bone Marrow-derived Cultured Mast Cells (BMCMCs)
03:48Engraftment of Mast Cell-deficient Mice with BMCMCs
05:30Peritoneal Mast Cell Engraftment Analysis
06:50Results: Representative BMCMC In Vitro Culture and In Vivo Engraftment
08:08Conclusion

Summary

Traduzione automatica

我们描述了体外衍生桅杆细胞的生成方法，它们被移植到乳腺细胞缺乏的小鼠中，以及不同解剖部位对网状桅杆细胞的表型、数量和分布的分析。此协议可用于评估桅杆细胞 在体内的功能。

Tags

Mast Cells In Vivo Knock-in Mice Bone Marrow-derived Cultured Mast Cells Adoptive Transfer Anatomical Distribution Host Defense Allergic Reactions

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