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Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing
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JoVE Journal Bioingegneria
Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

DOI:
10.3791/52754-v

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09:58 min

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April 23, 2015

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, , ,
1Department of Biomedical Engineering,The University of Akron

Capitoli

  • 00:05Titolo
  • 02:04Preparation of a Polymeric Aqueous Two Phase System
  • 03:31Printing of Tumor Spheroids into a 96-well Plate
  • 06:18Drug Treatment and Analysis of Viability in Cancer Cell Spheroids
  • 08:01Results: Narrow Spheroid Size Distribution Is Essential for Drug Testing
  • 09:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

A protocol for robotic printing of cancer cell spheroids in a high throughput 96-well plate format using an aqueous two-phase system is presented.

Tags

Keywords: Cancer Cell SpheroidsIn Vitro Tumor ModelAqueous Two-phase SystemHigh-throughput Drug TestingRobotic Liquid HandlingCell ViabilityCompound Screening

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