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Capitoli
- 00:00Titolo
- 01:34Dye Preparation
- 02:36Whole-mount Embryo Labeling
- 03:39Confocal Imaging
- 04:19Representative Results
- 05:15Conclusion
A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.
