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基于海拉细胞自由表达系统中的表达<em>疟原虫</em>棒状体蛋白
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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
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基于海拉细胞自由表达系统中的表达<em>疟原虫</em>棒状体蛋白

DOI:
10.3791/52772-v

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09:03 min

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June 10, 2015

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1Department of Biological, Geological, and Environmental Sciences,Cleveland State University

Capitoli

  • 00:05Titolo
  • 02:12Protein Expression Using In Vitro Human Cell Free Expression System
  • 03:21Purification of Expressed Recombinant Proteins
  • 05:05Western Blotting
  • 06:52Results: Purple Color Band on Nitrocellulose Paper During Developing Step Confirms the Successful Expression of Proteins
  • 08:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

基于细胞系统疟疾蛋白的表达仍然具有挑战性。我们证明两步并一步IVT（ 体外翻译）无细胞表达系统从HeLa细胞表达的重组疟疾蛋白质棒状体。我们使用的Ni树脂基于亲和性的纯化系统纯化棒状体蛋白质。

Tags

Keywords: HeLa Cell-free ExpressionPlasmodium ProteinsMalariaVaccine CandidatesCell-free Expression SystemsProtein ExpressionIn Vitro Human Cell-free ExpressionPF3D7 0114100PF3D7 0925900PF3D7 1361800Protein YieldAffinity Purification

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