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عالية الإنتاجية قياس خارج الخلية DNA الإصدار والكمي NET تشكيل في العدلات الإنسان<em> في المختبر</em
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
DOI:

11:03 min

June 18, 2016

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Capitoli

  • 00:05Titolo
  • 00:49Isolation of Neutrophils from Peripheral Human Blood
  • 02:17Measurement of Kinetics of Extracellular DNA Release Using Microplate Fluorimeter
  • 04:22Quantitation of NET Formation by MPO-DNA and HNE-DNA ELISA Assays
  • 08:22Results: MPO-DNA and HNE-DNA ELISA Assay Results
  • 10:25Conclusion

Summary

Traduzione automatica

High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.

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