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测量去泛素酶的活性细胞系和组织样品的方法

JoVE Journal
Biologia

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JoVE Journal Biologia

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

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测量去泛素酶的活性细胞系和组织样品的方法

DOI:

10.3791/52784-v

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09:45 min

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May 10, 2015

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Percy Griffin², Ashley Sexton, Lauren Macneill, Yoshie Iizuka⁴, Michael K. Lee², Martina Bazzaro⁴

¹Department of Neuroscience,University of Minnesota, ²Institute for Translational Neuroscience,University of Minnesota, ³Department of Obstetrics, Gynecology, and Women’s Heath,University of Minnesota, ⁴Masonic Cancer Center,University of Minnesota

Capitoli

00:05Titolo
01:09Culturing Cells for the Experiment
02:24Cell Harvesting and Cell Lysis
04:40Sample Derivatization
06:03Western Blot
07:42Results: Analysis of Deubiquinating Enzyme Activity
09:07Conclusion

Summary

Traduzione automatica

目前的协议细节，用于测量功能性同源去泛素酶的活性的方法。专门探针共价修饰的酶，并允许进行检测。这种方法保存，以确定新的治疗靶点的潜力。

Tags

Deubiquitinating Enzymes Ubiquitin-proteasome System Cell Lines Activity Assay Proteasomal Degradation Active Site Probes Enzyme Activity Expression Cell Disruption Pathologies Neurodegenerative Diseases Cancer

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