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Rose Bengal Photothrombosis door confocale Optical Imaging<em> In Vivo</em>: Een model van de Single Vessel Stroke
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Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke
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Rose Bengal Photothrombosis door confocale Optical Imaging<em> In Vivo</em>: Een model van de Single Vessel Stroke

DOI:
10.3791/52794-v

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08:07 min

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June 23, 2015

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, , , ,
1Department of Cellular and Structural Biology,The University of Texas Health Science Center San Antonio, 2School of Medicine,The University of Texas Health Science Center San Antonio, 3Cell & Tissue Imaging Center,St. Jude Children’s Research Hospital

Capitoli

  • 00:05Titolo
  • 01:11Preparation for the Surgery
  • 02:17Surgery
  • 03:43The Microscope Set Up
  • 04:30Utilizing Rose Bengal Dye for Stroke Induction
  • 06:37Results: Vessel Occlusion and Stroke Confirmation
  • 07:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.

Tags

Keywords: Rose Bengal PhotothrombosisConfocal Optical ImagingIn VivoSingle Vessel StrokeCranial WindowSomatosensory CortexMouse ModelLongitudinal StudiesIschemic EventLaser ExcitationPhotosensitive DyeConfocal MicroscopyIn Vivo Imaging

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